Superresolution Microscopy

Single molecule-based super-resolution microscopy

Overlay of a preSTORM and a STORM image of a T cell.

In ultra-high resolution Microscopy (PALM and STORM), we  image biological structures and processes at length scales far below the optical diffraction limit of a few hundred nanometers. In this project we address the technical limitations using (combinations of) new approaches, and we develop new holistic model-based data analysis strategies. We aim to achieve resolution of one nanometer by developing single particle analysis tools in order to make dynamic super-resolution imaging a valid method for studying mobile protein clusters in live cells.

Structural geometry and shape statistics

We construct reconstruction algorithms in ultra-high resolution microscopy for PALM and STORM imaging systems for the analysis of biosystems. Mathematically, the data are different modes of protomers. The techniques used here are methods from shape statistics and denoising. In order to reconstruct the statistics of the geometries of the oligomers in a cell, we have to  take into account their symmetries.

Research Team

From Subproject: Tomography with Uncertainties

University of Vienna
Computational Science Center
Oskar-Morgenstern-Platz 1
1090 Vienna, Austria
Otmar Scherzer Otmar Scherzer Principal Investigator
Professor of Mathematics
Head of the Computational Science Center
Ekaterina Sherina Ekaterina Sherina Postdoctoral Researcher

From Subproject: Ultra-high Resolution Microscopy

Vienna University of Technology
Institute of Applied Physics – Biophysics
Getreidemarkt 9
1060 Vienna, Austria
Gerhard Schütz Gerhard Schütz Principal Investigator
Professor at the Institute of Applied Physics
Montse López Montse López Postdoctoral Researcher
Magdalena Schneider
Magdalena Schneider
PhD student